Composite

Part:BBa_K4444004

Designed by: Adiva Daniar, Shreya Vallimanalan   Group: iGEM22_UPenn   (2022-10-10)
Revision as of 03:16, 11 October 2022 by Registry (Talk | contribs)

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PhoCl Display GFP

This is a modified backbone from the RELEASE plamid. It does not contain a furin cut site. This backbone contains GFP, a tri-transmembrane domain, and ER retention motif. The plasmid uses ampicillin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 898
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 1543


[edit]
Categories
Parameters
None