Composite

Part:BBa_K4509569

Designed by: MARIE JYOTSNA JAWAHAR, Vibha Murali, Rhea Harry William   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 14:18, 28 September 2022 by Marie JJ (Talk | contribs)


HORSERADISH PEROXIDASE with constitutive promoter J23100

The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms and the most studied type is C. The composite part starts with the J23100 constitutive promoter, continues with RBS and is followed by the Horseradish Peroxidase coding sequence. The sequence ends with a terminator and a BamH1 restriction site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1022
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None