Composite
Part:BBa_K4385020
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-06)
Zinc-inducible surface display
This part is composed of ZraSR two-component signal transport system, PzraP and OmpC-ZBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PzraP promoter promotes the transcription of downstream OmpC-ZBP, and realizes the adsorption of zinc ions in the environment. Add 6*Hig Tag to this sequence to purify recombinant protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 616
Illegal SpeI site found at 775
Illegal PstI site found at 1123 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 616
Illegal NheI site found at 1506
Illegal NheI site found at 1529
Illegal SpeI site found at 775
Illegal PstI site found at 1123 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 616
Illegal BglII site found at 324
Illegal BglII site found at 1641 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 616
Illegal SpeI site found at 775
Illegal PstI site found at 1123 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 616
Illegal SpeI site found at 775
Illegal PstI site found at 1123
Illegal AgeI site found at 442 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |