Composite

Part:BBa_K4166014

Designed by: Alexa DeKorte, Christina Harbin   Group: iGEM22_Alma   (2022-10-11)
Revision as of 16:20, 11 October 2022 by Registry (Talk | contribs)

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This is a control for our kill switch which is activated at low AHL concentrations

In this control, RFP takes the place of MazF, a toxin which kills the cells, and GFP takes the place of MazE, the anti-toxin. By measuring the relative amounts of each fluorescent protein, we can estimate how effective the kill switch should be.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 918
    Illegal NheI site found at 941
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 331
    Illegal AgeI site found at 3035
    Illegal AgeI site found at 3147
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None