Composite
Part:BBa_K4218005
Designed by: Wuhui Jiang Group: iGEM22_YiYe-China (2022-10-08)
The dual luciferase reporter system
According to previous studies, we found that exons of zinc finger protein 91 (ZFN91) was skipped in MDS patients. This mechanism gives rise to our idea of constructing the sensor based on the exon skipping of ZFN91 to the plasmid containing reporter genes, which monitor the alteration of RNA splicing in cells. Our project will help us to evaluate the effectiveness of these sensors in diagnosing MDS patients in the future.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2496
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3698
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Categories
Parameters
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