Part:BBa_K4438709
P4G13_trigger_5_phi29
P4G13_trigger_5_phi29 (BBa_K4438709) is single-stranded DNA having 26 nucleotides . Figure 1B) shows the secondary structure and minimum free energy.
Usage and Biology
The part P4G13_trigger_5_phi29 (BBa_K4438709) is complementary to the part P4G13_aptamer (BBa_K4438700) with few mismatches. It blocks the entire binding region of the progesterone aptamer [1]. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Progesterone binds with P4G13_aptamer (BBa_K4438700) with high affinity and displaces the P4G13_trigger_5_phi29 (BBa_K4438709) [2]. This part has complete complementarity with part P4G13_Target_5 (BBa_K4438710) . Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of progesterone can be detected using all these three parts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Alhadrami, H. A., Chinnappan, R., Eissa, S., Rahamn, A. A., & Zourob, M. (2017). High affinity truncated DNA aptamers for the development of fluorescence based progesterone biosensors. Analytical biochemistry, 525, 78-84.
Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.
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