Composite

Part:BBa_K4229018

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)
Revision as of 11:43, 11 October 2022 by NikitaEdel (Talk | contribs)


RBS-spyCatcherXiaI-RBS-TnaB

These are the genes we put into MCS2. The first one is XiaI fused with the spycatcher(BBa_K4229015) and the second is the L-tryptophan transport protein TnaB(BBa_K4229003). It was decided to put the enzymes with the catcher at the beginning of each MCS as those are our most important proteins, which we wanted to have produced the most. These two genes build together with the Biobrick BBa_K42216 all enzymes of the indigo/indirubin pathway. The production was tested under the T7 promotor and LacI operator in those two Biobricks: BBa_K4229033 and BBa_K4229034)

Grafik indigo weier bg.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 893
    Illegal AgeI site found at 783
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None