Composite

Part:BBa_K4160013

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)
Revision as of 20:08, 9 October 2022 by Registry (Talk | contribs)

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GEMS receptor construct containing PR3 fused to HA-tag as affinity domain with 8 amino acid linker

This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002, BBa_K4160004, BBa_K1150016, and BBa_K2217015 containing a linker of 8 amino acids.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2390
    Illegal PstI site found at 191
    Illegal PstI site found at 1078
    Illegal PstI site found at 2073
    Illegal PstI site found at 2234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 760
    Illegal NheI site found at 1447
    Illegal PstI site found at 191
    Illegal PstI site found at 1078
    Illegal PstI site found at 2073
    Illegal PstI site found at 2234
    Illegal NotI site found at 2377
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 760
    Illegal BglII site found at 1551
    Illegal BglII site found at 1737
    Illegal BglII site found at 2001
    Illegal BamHI site found at 64
    Illegal XhoI site found at 961
    Illegal XhoI site found at 2384
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2390
    Illegal PstI site found at 191
    Illegal PstI site found at 1078
    Illegal PstI site found at 2073
    Illegal PstI site found at 2234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2390
    Illegal PstI site found at 191
    Illegal PstI site found at 1078
    Illegal PstI site found at 2073
    Illegal PstI site found at 2234
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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