Coding

Part:BBa_K4165255

Designed by: Esraa Elmligy   Group: iGEM22_CU_Egypt   (2022-10-08)
Revision as of 19:44, 11 October 2022 by SalmaMohsen 2000 (Talk | contribs)


GST-Coh2-G4S- TD28REV

Long description

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 689
    Illegal SapI.rc site found at 85

WetLab Results

Transformation of GST COH (L) TD28 Rev in BL-21 using pGS-21a vector 

                                Figure 1. Transformed plate of GST COH (L) TD28 Rev + pGS-21a

Transformation of GST COH (L) TD21 Rev in DH-5 alpha using pJET vector 

                               Figure 2. Transformed plate of GST COH (L) TD28 Rev + pJET

Comparison between chemical lysis and sonication for GST COH TD28 Rev

Figure 3. this graph shows a difference between chemical lysis and sonication for GST COH TD28 Rev, after we had the results we optimized our protocol to use chemical lysis for GST COH (L) TD28 Rev

SDS PAGE of induced and non induced samples of GST COH TD28 Rev

Figure 4. This figure shows the comparison between the induced and non induced samples of GST COH TD28Rev, where well no.2 is the induced sample of GST COH TD28 Rev while well no.6 is the non induced sample of GST COH TD28Rev showing that the induction successfully happened owing to the IPTG

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Parameters
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