Coding

Part:BBa_K4439007

Designed by: Charlotte Daumal   Group: iGEM22_EPFL   (2022-09-29)
Revision as of 22:06, 11 October 2022 by Charlottedml (Talk | contribs)

mSA-N[AS]4C-CBD-10xHis


Abstract

To complete

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Protein's Characterization

Description

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Usage and Biology

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Modeling

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Experiments

Adapted Protocols

Protein Purification using Ni-NTA Beads

Materials . 2.5M imidazole stock solution . 5M NaCl solution . Protease inhibitor tablet . Turbonuclease . Sonicator . 1M HEPES . HisPur Ni-NTA beads in 20% ethanol . Disposable plastic column . Centrifuge going to 20 000 xg . 0.30 µm filters . Tube of bacterial culture induced with IPTG



Lab's Tips and Tricks

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Results

Expression

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Purification

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Biofilm Fabrication

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Hydrophobicity tests

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References

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Categories
Parameters
None