Coding

Part:BBa_K4229001

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)
Revision as of 15:52, 9 October 2022 by NikitaEdel (Talk | contribs)


Fre (NAD(P)H-flavin reductase)



Fre is one of our four enzymes we used in our project to produce indigo and indirubin. Fre catalyzes the reduction of soluble flavins by reduced pyridine nucleotides (UniProt ID:P0AEN1). In our pathway it accelerates the recycling of the cofactor NADPH, adn adds more FADH2 for the enzyme XiaI.

Grafik indigo weier bg.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 304
    Illegal SapI.rc site found at 424


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Categories
Parameters
None