Part:BBa_K4144010

Improved recombined SAMe transporter opPet8p fused with linker

Usage and Biology

Linkers can be classified into three groups: flexible, rigid and cleavable. Flexible linkers are generally composed of small, non-polar or polar residues such as Gly, Ser and Thr. The most common is the (Gly4Ser)n linker (Gly–Gly–Gly–Gly–Ser)n, where n indicates the number of repeats of the motif. Polyglycine linkers have also been evaluated, but the addition of a polar residue such as serine can reduce linker–protein interactions and preserve protein function. Due to their flexibility, these linkers are unstructured and thus provided limited domain separation in a previous study.

We WHU-China plan to use such linker to contact opPet8p and sfGFP in order to avoid the opPet8p membrane localization disruption by adding a sfGFP tag.

Figure. 1 Linker addition design into opPet8p::sfGFP recombined protein

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 520
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 390
  Illegal BamHI site found at 1018
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1642
  
• 1000
  COMPATIBLE WITH RFC[1000]