Coding

Part:BBa_K4335003:Design

Designed by: Yuning Luo   Group: iGEM22_UESTC-BioTech   (2022-09-30)
Revision as of 17:25, 30 September 2022 by Registry (Talk | contribs)

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ChlamyHgR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 782
    Illegal NgoMIV site found at 890
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to make it easier to heterologously express in Chlamydomonas reinhardtii, we have optimized the codon and added a segment of the intron.


Source

Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29, 2498-2518. Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., et al. (2022). A highly photostable and bright green fluorescent protein. Nat Biotechnol 40, 1132-1142.

References