Coding

Part:BBa_K4169000

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-29)
Revision as of 02:41, 30 September 2022 by Registry (Talk | contribs)

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Nattokinase gene with pelB

Signal peptide gene of PelB can direct aprN to Escherichia coli periplasmic membrane. The propeptide has been proved to function as an intramolecular chaperone and is essential for guiding the correct folding of the linked protein to its mature form. The nattokinase has potent fibrinolytic activity, which could enhance and prolong fibrinase in the plasma. Based on its food origin and relatively strong fibrinolytic activity, it a possible safe and efficient medicine for use in the prevention of thrombosis diseases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 202
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
biology