Plasmid

Part:BBa_K4252023

Designed by: Jiajie Zhang   Group: iGEM22_HNU_China   (2022-10-11)
Revision as of 09:48, 11 October 2022 by Registry (Talk | contribs)

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cI857-R promoter-phlF-pst plasmid

cI857-R promoter-phlF-pst plasmid involved: The temperature-sensitive control element cI857(BBa_K200011) R promoter, phlF repressor(BBa_K1725040) pst(PstS:BBa_K4252005;PstC BBa_K4252006;PstA: BBa_K4252007;PstB: BBa_K4252008;PstSCAB:BBa_K4252009). We use different temperatures to culture the strain. It is estimated that cI857 forms dimer under 30℃ culture conditions to inhibit the R promoter, so that its downstream phlf and pst do not express. The cI857 dimer was depolymerized at 37 ℃, and the inhibition of the R promoter was relieved. The downstream phlf and pst were normally expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2251
    Illegal AgeI site found at 4166
    Illegal AgeI site found at 5180
    Illegal AgeI site found at 5369
    Illegal AgeI site found at 5935
    Illegal AgeI site found at 7936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5000
    Illegal SapI.rc site found at 8952


[edit]
Categories
//classic/signalling/receiver
//function/sensor
Parameters
abs
function
switch_point