Coding
Part:BBa_K4117222:Design
Designed by: He Du,Yue Zhang Group: iGEM22_BNU-China (2022-09-30)
UGT1A3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1266
Illegal BamHI site found at 453 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 40
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since this subunit has been proved to be expressed successfully, we did not re-engineer this protein gene.
Source
We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503