Composite

Part:BBa_K4509420:Design

Designed by: Vibha Murali, MARIE JYOTSNA JAWAHAR, Rhea Harry William   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 15:27, 11 October 2022 by Marie JJ (Talk | contribs) (→‎Characterization)


HORSERADISH PEROXIDASE with constitutive promoter J23116


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1022
  • 1000
    COMPATIBLE WITH RFC[1000]


Source and Improvement

The promoter J23119 is replaced with J23116. This switch in the design will reduce the burden in the cell during overexpression of enzymes

Characterization

File:K4509420 graph1.jpg

References