Coding

Part:BBa_K258101:Design

Designed by: Cihan Tastan   Group: iGEM09_METU-Gene   (2009-10-10)
Revision as of 22:34, 10 October 2009 by Registry (Talk | contribs)

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PrtE domain of ABC transporter of Erwinia chrysanthemi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1136
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1136
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene actually will be fully function with other two genes which are PrtD and PrtF.


Source

E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 50 kd protein to the prtD gene product (protein PrtD).

References