Reporter

Part:BBa_K4365003

Designed by: Giorgio Gilioli   Group: iGEM22_TU_Dresden   (2022-09-30)
Revision as of 12:59, 30 September 2022 by Registry (Talk | contribs)

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turboRFP Selection Device with constitutive promoter

Improvement of BBa_J04450 part. In the original construct, mRFP1 expression was observed irrespective of IPTG induction. So we replaced the inducible LacI-regulated promoter (BBa_R0010) with a constitutive (BBa_J23100) for colony screening.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 683


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