Device

Part:BBa_K174007:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-10)
Revision as of 19:20, 10 October 2009 by Registry (Talk | contribs)

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Degradation controller with integration site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To test the integration, pmutin4 integration vector is selected. However after the integration pspac is left on the right side, free to drive the expression of downstream genes. To remove the effect of pspac promoter of pmutin4 vector, the device with ligation of sac integration site, araR repressible promoter and sspB CDS was amplified with PacI from one end. Hence by cutting pmutin4 with PacI, pspac promoter can be removed. For the other end, sacII was selected since it is compatible with PacI. PacI is just on the left side of Terminator0 on pmutin4, it will also be removed leaving Terminator 1 and Terminator 3 on the vector.


Source

Ligated manually

References