Composite

Part:BBa_K4204020:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-11)
Revision as of 14:58, 11 October 2022 by Jianfei Song (Talk | contribs) (→‎Source)


Crystal violet induced GFP improved with ProQC system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1166
    Illegal NheI site found at 1248
    Illegal NheI site found at 2332
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1045
    Illegal BamHI site found at 1943
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
    Illegal NgoMIV site found at 1995
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initial design of the part has a flaw (a gap between the RBS and the start codon leads to a frameshift of the resulting protein). This problem is fixed now by another round of design, build and test.

Source

The protein quality control system is from Yang et al's work [1], the crystal violet induction system is from 's work [2]. Other components are from BBa_K653003.Assembled by BNDS_China 2022.

References