Regulatory
Part:BBa_K4365002:Design
Designed by: Giorgio Gilioli Group: iGEM22_TU_Dresden (2022-09-29)
2 micron Ori
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 360
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 360
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 360
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Was amplified using a forward primer containing the BsmBI restriction site and a reverse primer containing the BsrBI restriction site
Source
p426 Mumberg plasmid