Composite

Part:BBa_K4204020:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-11)
Revision as of 14:40, 11 October 2022 by Jianfei Song (Talk | contribs) (→‎Design Notes)


Crystal violet induced GFP improved with ProQC system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1166
    Illegal NheI site found at 1248
    Illegal NheI site found at 2332
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1045
    Illegal BamHI site found at 1943
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
    Illegal NgoMIV site found at 1995
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initial design of the part has a flaw (a gap between the RBS and the start codon leads to a frameshift of the resulting protein). This problem is fixed now by another round of design, build and test.

Source

The protein quality control system is from 's work, the crystal violet induction system is from 's work. Other components are from BBa_K653003.Assembled by BNDS_China 2022.

References