Plasmid
CueO-PET28

Part:BBa_K4232008

Designed by: Songyuan Kong   Group: iGEM22_BIT-China   (2022-10-03)
Revision as of 14:26, 11 October 2022 by Eatting (Talk | contribs)


A plasmid used to catalyze oxygen and reducing substances

BIT-China-parts-Protein structure of laccase.jpeg

This is a plasmid made of laccase with pet28a for expression in the introduction of E. coli.The protein structure of the laccase is shown in the figure on the right. Laccase, known as multicopper oxidase CueO,The specific working principle is shown in theBBa_K4232000.








Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1751
    Illegal XbaI site found at 1894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1751
    Illegal NheI site found at 1790
    Illegal NotI site found at 1724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1751
    Illegal BglII site found at 1960
    Illegal BamHI site found at 1757
    Illegal XhoI site found at 1717
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1751
    Illegal XbaI site found at 1894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1751
    Illegal XbaI site found at 1894
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 1992
    Illegal NgoMIV site found at 3580
    Illegal NgoMIV site found at 3740
    Illegal NgoMIV site found at 6783
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4657


[edit]
Categories
Parameters
None