Composite

Part:BBa_K4156113

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 07:09, 9 October 2022 by Liwei (Talk | contribs)


plac-mRFP

plac-mRFP is a biobrick that expresses mRFP controlled by the plac operon ( BBa_R0010 ). plac-mRFP functions as a red fluorescent signal to characterize the performance of the plac operon


Usage and Biology

In this biobrick, we used the plac operon to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. this design allowed the spatiotemporal intensity of plac manipulator-regulated gene expression to be measured by the red fluorescent signal. We were thus able to verify the specific response of the plac operon to lactose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 864
    Illegal AgeI site found at 976
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None