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Part:BBa_K258006:Design

Designed by: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology   Group: iGEM09_METU-Gene   (2009-10-09)
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Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was optimized for E.coli with Biobrick restriction sites


Source

The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter.

References