Tag
Part:BBa_K258006:Design
Designed by: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology Group: iGEM09_METU-Gene (2009-10-09)
Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 651
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was optimized for E.coli with Biobrick restriction sites
Source
The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter.