Composite

Part:BBa_K4229016

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)
Revision as of 11:30, 11 October 2022 by NikitaEdel (Talk | contribs)


RBS-snoopCatcherTnaA-RBS-FRE

These are the genes we put inside MCS1 of our vector pCDF-Duet-1. The first gene is the fusion protein of TnaA with the snoopcatcher(BBa_K4229011) on the C-terminal, the second one being Fre(BBa_K4229001). It was decided to put the enzymes with the catcher at the beginning of each MCS as those are our most important proteins, which we wanted to have produced the most. These two genes build together with the Biobrick BBa_K42218 all enzymes of the indigo/indirubin pathway. The production was tested under the T7 promotor and LacI operator in those two Biobricks: BBa_K4229033 and BBa_K4229034)

Grafik indigo weier bg.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 990
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 990
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 990
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 990
    Illegal AgeI site found at 155
    Illegal AgeI site found at 241
    Illegal AgeI site found at 1613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2150
    Illegal SapI.rc site found at 2270


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Categories
Parameters
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