Plasmid_Backbone

Part:BBa_K4365022

Designed by: Giorgio Gilioli   Group: iGEM22_TU_Dresden   (2022-09-30)
Revision as of 19:46, 30 September 2022 by Registry (Talk | contribs)

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pSB1KY-URA3

backbone for shuttle vector

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4632
    Illegal XbaI site found at 473
    Illegal SpeI site found at 1481
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4632
    Illegal SpeI site found at 1481
    Illegal PstI site found at 12
    Illegal NotI site found at 2554
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4632
    Illegal BamHI site found at 108
    Illegal XhoI site found at 3482
    Illegal XhoI site found at 4508
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4632
    Illegal XbaI site found at 473
    Illegal SpeI site found at 1481
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4632
    Illegal XbaI site found at 473
    Illegal SpeI site found at 1481
    Illegal PstI site found at 12
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 4638
    Illegal SapI.rc site found at 5


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Parameters
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