Part:BBa_K4361300

BlcR D37R

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R1 (Part:BBa_K4361200) and F1.1 D37R (Part:BBa_K4361201). For this mutant, the aspartic acid in position 37 has been changed to arginine by mutating the GAC codon to CGC.

This mutant also contains the following nucleotide mutations outside of the targeted site:

• G 763 > A, resulting in substitution E255K

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 694
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 78
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 589
  

Usage and Biology

Figure 1. SDS PAGE gel of PURE reactions with GreenLys (fluorescent Cy2-labeled lysine) and a variant of Part:BBa_K4361106 containing a BlcR mutant (this part through Part:BBa_K4361319). This part corresponds to lane 1.