Composite

Part:BBa_K4218002

Designed by: Wuhui Jiang   Group: iGEM22_YiYe-China   (2022-09-30)
Revision as of 07:10, 30 September 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


This part is composite of T7 promoter, double toehold switch part BBa_K3822000,FP mcherry and termin

In our project, the trigger sequence is linked to DNA fragment of biomarker by DNA amplification and then was constructed into a plasimd. After con-transformation into E.coli with the plasmid contain double toehold sequence, the biomarker RNA will also be connected with trigger. The product with trigger can open the stem-loop structure and form a red fluorescent protein, which is the visible observation result.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None