Composite

Part:BBa_K4335022

Designed by: Hang Zhang   Group: iGEM22_UESTC-BioTech   (2022-10-10)
Revision as of 09:28, 11 October 2022 by ZXFeng (Talk | contribs)


Rbcs2 Promotor+ChlamyHgR+Rbcs2 Term

BBa K4335022 consists of a promoter, a resistance gene, and a terminator. The resistance gene APHVII[BBa_K4335003] was optimized for Chlamydomonas reinhardtii on the basis of the progene. We inserted a 145 BP intron into the progene to facilitate its expression in Chlamydomonas reinhardtii. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1121
  • 1000
    COMPATIBLE WITH RFC[1000]


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