Part:BBa_K4252009

Phosphate-specific transportor (Pst)

The phosphate (Pi)-specific transport system of Escherichia coli (Pst) is a typical ABC transport system composed of four different proteins: PstS, the periplasmic Pi-binding protein; PstC and PstA, integral membrane proteins that mediate the translocation of Pi through the inner membrane and PstB that binds ATP and energizes the transport. The operon that encodes Pst contains five genes in the following order: pstS, pstC, pstA, pstB, and a fifth distal gene, phoU, whose product does not play a role in the transport of Pi. The Pst system encodes an ATPbinding cassette (ABC) transporter involved in the transport of inorganic phosphate (Pi). As a member of the PHO regulon, the Pst operon is induced in response to Pi limitation. In order to allow E. coli to absorb more Pi, we link the Pst (only contains pstS, pstC, pstA, pstB) gene onto the expression vector pET-28a(+) and introduced it into E.coli BL21. Through inducing its expression by IPTG, E. coli BL21 can efficiently absorb Pi when the external Pi isn’t in limitation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 154
  Illegal AgeI site found at 720
  Illegal AgeI site found at 2721
  Illegal AgeI site found at 3917
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3737