Primer

Part:BBa_K4361113

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-08)
Revision as of 08:45, 11 October 2022 by Larsvdb (Talk | contribs)


Blc operator SDM primer R

A site-directed mutagenesis primer, to be used in conjunction with the mutation-inducing forward primer Part:BBa_K4361112. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert (Part:BBa_K4361115), a deletion will be induced in the operator, resulting in the altered sequence as described in Part:BBa_K4361114. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence (Part:BBa_K4361114) may act as a negative control when testing the binding properties of BlcR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None