Composite

Part:BBa_K4297086

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)
Revision as of 15:06, 10 October 2022 by Registry (Talk | contribs)

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PL-CBE-pET-Alloferon-1-eGFP-SD-AmpR

The PL-CBE and the optimized pET plasmid were fused to facilitate the subsequent construction of the RBS library.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2097
    Illegal NheI site found at 6019
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5432
    Illegal BamHI site found at 4376
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5111
    Illegal AgeI site found at 5757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 988
    Illegal BsaI site found at 5114
    Illegal BsaI site found at 5760
    Illegal BsaI site found at 7055
    Illegal BsaI site found at 7781
    Illegal BsaI.rc site found at 5108
    Illegal BsaI.rc site found at 5754
    Illegal BsaI.rc site found at 6028
    Illegal BsaI.rc site found at 7932


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Categories
Parameters
None