Regulatory
Part:BBa_K4317001
Designed by: Chris Song Group: iGEM22_Seoul_Korea (2022-10-11)
P2069M
P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis.
Microorganisms 2020, 8, 1030; doi:10.3390/microorganisms8071030
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal BglII site found at 13
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |