Part:BBa_K4182011:Design

Temperature regulated suicide circuit

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1018
  Illegal suffix found in sequence at 1446
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1018
  Illegal SpeI site found at 1447
  Illegal PstI site found at 1461
  Illegal NotI site found at 1024
  Illegal NotI site found at 1454
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1018
  Illegal XhoI site found at 2478
  Illegal XhoI site found at 3504
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1018
  Illegal suffix found in sequence at 1447
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1018
  Illegal XbaI site found at 1033
  Illegal SpeI site found at 1447
  Illegal PstI site found at 1461
  
• 1000
  COMPATIBLE WITH RFC[1000]

Profile

Design Notes

This gene has been optimized for E. coli

Source

Escherichia phage phiX174(from https://www.ncbi.nlm.nih.gov/gene/2546400)

References

1. Din, M.O., et al., Synchronized cycles of bacterial lysis for in vivo delivery. Nature, 2016. 536(7614): p. 81-85.

2. Saeidi, N., et al., Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Mol Syst Biol, 2011. 7: p. 521.

3. Restrepo-Pineda, S., et al., Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights. FEMS Microbiol Rev, 2021. 45(6).

4. Aparicio, T., V. de Lorenzo, and E. Martínez-García, Improved Thermotolerance of Genome-Reduced Pseudomonas putida EM42 Enables Effective Functioning of the PL/cI857 System. Biotechnology Journal, 2019. 14(1): p. 1800483.