Reporter
Part:BBa_K4335002
Designed by: Yuning Luo Group: iGEM22_UESTC-BioTech (2022-09-30)
StayGold, a newly discoverd green fluorescent protein
StayGold, a green fluorescent protein (GFP) , fluoresces in expression. StayGold has an order of magnitude more photostability than any currently available fluorescent protein. Commonly used to label and trace cellular components. BBa_K4335002 is our StayGold gene optimized for the codon of Chlamydomonas reinhardtii.
Additional Materials
StayGold was extracted from Cytaeis uchidae by Atsushi Miyawaki et al. by the RIKEN Center for Brain Science Research on April 25, 2022. StayGold has an order of magnitude more light stability than any currently available fluorescent protein and has a cell brightness similar to that of mNeonGreen. Compared with the 15 fluorescent proteins, StayGold showed good photostability.Usage
We introduced the StayGold gene into the pTX2040 plasmid. PCrRBCS2 is used as the promoter and TCrRBCS2 is used as the terminator to achieve the expression of the StayGold gene.Result
Plasmid construction
We designed two primers: Staygold-F and Staygold-R, and confirmed the successful assembly by PCR amplification.Functional Identification
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 540
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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