Reporter

Part:BBa_K4335002

Designed by: Yuning Luo   Group: iGEM22_UESTC-BioTech   (2022-09-30)
Revision as of 17:11, 30 September 2022 by Registry (Talk | contribs)

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StayGold, a newly discoverd green fluorescent protein

Here we present StayGold, a green fluorescent protein (GFP) derived from the jellyfish Cytaeis uchidae. StayGold is over one order of magnitude more photostable than any currently available fluorescent protein and has a cellular brightness similar to mNeonGreen. Researchers used StayGold to image the dynamics of the endoplasmic reticulum (ER) with high spatiotemporal resolution over several minutes using structured illumination microscopy (SIM) and observed substantially less photobleaching than with a GFP variant optimized for stability in the ER. StayGold will substantially reduce the limitations imposed by photobleaching, especially in the live cell or volumetric imaging. We chose StayGold as our reporter fluorescent protein because it has an order of magnitude greater photostability than any currently available fluorescent protein, and the excitation and emission wavelengths of Staygold do not overlap with the chloroplast fluorescence of Chlamydomonas reinhardtii itself

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 540
  • 1000
    COMPATIBLE WITH RFC[1000]


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