Composite

Part:BBa_K4340605

Designed by: Chein Yueh Liu   Group: iGEM22_PuiChing_Macau   (2022-09-29)
Revision as of 02:06, 30 September 2022 by Registry (Talk | contribs)

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sfGFP

sfGFP(BBa_K2762014) is able to show fluorescence in acidic environment. We use this plasmid to test the growth curve in our hydroponic system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1283
    Illegal PstI site found at 5809
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1238
    Illegal PstI site found at 5809
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1349
    Illegal BamHI site found at 1205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1283
    Illegal PstI site found at 5809
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1283
    Illegal PstI site found at 5809
    Illegal NgoMIV site found at 1381
    Illegal NgoMIV site found at 2969
    Illegal NgoMIV site found at 3129
    Illegal NgoMIV site found at 3483
    Illegal AgeI site found at 752
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5635
    Illegal SapI site found at 4552


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Categories
Parameters
None