Plasmid

Part:BBa_K4182009:Design

Designed by: Boyang Zhou   Group: iGEM22_XJTU-China   (2022-10-10)
Revision as of 01:30, 11 October 2022 by Dan012 (Talk | contribs) (→‎Design Notes)


A circuit for efficient exopolysaccharide synthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4724
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4724
    Illegal BglII site found at 4733
    Illegal BglII site found at 7225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4724
    Illegal NgoMIV site found at 1103
    Illegal NgoMIV site found at 4610
    Illegal AgeI site found at 6785
    Illegal AgeI site found at 6951
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Base Pairs

6356

Design Notes

we found that using the pSEVA341 plasmid Replacing the medium-high copy pSB1K3 plasmid as a vector resulted in a higher degree of expression as well as a more significant stability of gene expression The pSEVA plasmid allows direct binding to express heterologous genes and retains polyclonal sites after binding to any gene. This modularity and compatibility with various replicons allows the assembly of complex circuits in the same host, and the ease of monitoring and modular control of each subcircuit helps ease the transition from trial-and-error genetic engineering to systematic synthetic biology. It is more beneficial for the characterization and practical application of modular research in synthetic biology. [2]We also designed the introduction of LacI regulatory protein to obtain the target gene expression product more accurately and efficiently, and to achieve better ability to regulate the product to achieve water fixation and moisture retention.

Source

E.coli

References