Plasmid

Part:BBa_K4182010:Design

Designed by: YiBo Yuan   Group: iGEM22_XJTU-China   (2022-10-10)
Revision as of 01:25, 11 October 2022 by Dan012 (Talk | contribs) (→‎Design Notes)


Critical synthesis circuitry from important precursor GPP to AA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal PstI site found at 5844
    Illegal PstI site found at 6024
    Illegal PstI site found at 7131
    Illegal PstI site found at 8462
    Illegal PstI site found at 10270
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal PstI site found at 5844
    Illegal PstI site found at 6024
    Illegal PstI site found at 7131
    Illegal PstI site found at 8462
    Illegal PstI site found at 10270
    Illegal NotI site found at 2615
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal BamHI site found at 5902
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal PstI site found at 5844
    Illegal PstI site found at 6024
    Illegal PstI site found at 7131
    Illegal PstI site found at 8462
    Illegal PstI site found at 10270
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal PstI site found at 5844
    Illegal PstI site found at 6024
    Illegal PstI site found at 7131
    Illegal PstI site found at 8462
    Illegal PstI site found at 10270
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 5610
    Illegal NgoMIV site found at 7733
    Illegal NgoMIV site found at 9063
    Illegal AgeI site found at 183
    Illegal AgeI site found at 7888
    Illegal AgeI site found at 9688
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Design Notes

The necessary E.coli. codon optimizations were made.

Source

LacI:E.coli FPPS:Rhodobacter azotoformans fpps gene for farnesyl diphosphate synthase, partial cds(GenBank: AB053174.1)

References