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Part:BBa_K4245201

Designed by: Michelle Jing, Daeun Lee, Richard Jiang, Sishnukeshav Balamurali, Christina Yi, Janet Standeven   Group: iGEM22_Lambert_GA   (2022-10-09)
Revision as of 17:30, 10 October 2022 by Sriragt (Talk | contribs)


hsa-miR-1-3p RCT Padlock Probe

This part is the sequence for the hsa-miR-1-3p RCT Padlock Probe, which our team designed to be specific to BBa_K4245006, hsa-miR-1-3p. This miRNA acts as a biomarker for Coronary Artery Disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Transcription approach, the produced transcript contains repeats of the middle sequence (hsa-miR-1-3p, the broccoli fluorescent RNA aptamer, and the padlock spacer sequence). We cloned the sequence into a pSB1K3 backbone.

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