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Part:BBa_K4245201:Experience

Designed by: Michelle Jing, Daeun Lee, Richard Jiang, Sishnukeshav Balamurali, Christina Yi, Janet Standeven   Group: iGEM22_Lambert_GA   (2022-10-09)
Revision as of 13:42, 10 October 2022 by Daeunlee (Talk | contribs)

Rolling Circle Transcription (RCT) was run with this part but proved to be unsuccessful. The products of RCT are long RNA strands composed of repeating complementary sequences of the used padlock probe. Therefore, one way in which the success of RCT can be determined is by running the RCT products on an agarose gel, since a fluorescent band very close to the wells would indicate the presence of extremely long nucleic acid strands. However, the products of RCT did not appear on a 1% agarose gel (see Fig. 1).


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