DNA
Part:BBa_K4342000
Designed by: Jeffrey Chuong and Samer Salman Group: iGEM22_Austin_UTexas (2022-10-02)
tdk/kan Cassette
Confers kanamycin resistance and AZT susceptibility. Designed to ligate to Gene Upstream Homology (Type 1) and Gene Downstream Homology (Type 5) via BsaI digestion for ADP1 Genetic Engineering. Used to ligate to P. destructans Target Sequence Upstream Homology (Type 1) and CymR Repressor (Type 56) via BsaI digestion in P. destructans Detector.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 719
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 713
Illegal XhoI site found at 875 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 719
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 719
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |