Protein_Domain

Part:BBa_K4375011

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-28)
Revision as of 09:26, 10 October 2022 by Golaritsagnes (Talk | contribs)


Llama immunoglobulin IgA hinge linker

It is a natural llama hinge region to provide a flexible linker for Nanobody and the protein fused to it. The amino acid sequence is STPPTPSPSTPP.

Usage and Biology

This region in the antibody ensures that the variable regions can move in space and thus bind to their antigen. Since it fulfills this role in nature, it is suitable for conjugation to nanobodies. This way the Nanobody remains rigid and can attach to the chosen antigen after conjugation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Li D, Ren J, Ji F, Peng Q, Teng H, Jia L. Peptide linker affecting the activity retention rate of VHH in immunosorbents. Biomolecules. 2020 Nov 27;10(12):1610.


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