Coding

Part:BBa_K4335013

Designed by: Hang Zhang   Group: iGEM22_UESTC-BioTech   (2022-10-10)
Revision as of 09:30, 10 October 2022 by Registry (Talk | contribs)

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SpCas9

The Cas9 protein is part of the bacteria's CRISPR/Cas9 immune defense mechanism to identify and destroy foreign DNA. By incorporating the foreign DNA into the bacteria's own DNA, it has a memory of any prior foreign DNA that the bacteria has encountered.The Cas9 endonuclease can be used either with the tracrRNA and crRNA (CRISPR RNA- Clustered Regularly Interspaced Short Palindromic Repeats) or with a guide RNA to cleave foreign DNA at a species locations. This makes exceptionally useful for DNA cloning in synthetic biology.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 241
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 241
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
    Illegal BglII site found at 1043
    Illegal BglII site found at 1733
    Illegal BglII site found at 3812
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 241
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 241
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
    Illegal NgoMIV site found at 1075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3468


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