Part:BBa_K4414005

GFP

Usage and Biology

There is an extensive use of fluorescent bioimaging in the fields of biochemistry, biotechnology, cell and developmental biology. In comparison with other fluorescent tags, GFP-like proteins have the advantage of forming internal chromophore without requiring accessory cofactors, enzymes or substrates other than molecular oxygen, making possible chromophore formation in live organisms, tissues and cells.[1][2].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional characterization

We connect GFP as a reporter gene to other sequences encoding proteins, as shown in the figure is the fluorescence image of GFP.

Figure 1.The picture of fluorescence diagram

Reference

[1].Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A., & Tsien, R. Y. (1995). Understanding, improving and using green fluorescent proteins. Trends in Biochemical Sciences, 20(11), 448–455. https://doi.org/10.1016/s0968-0004(00)89099-4.

[2].Stepanenko, O. V., Verkhusha, V. V., Kuznetsova, I. M., Uversky, V. N., & Turoverov, K. K. (2008). Fluorescent Proteins as Biomarkers and Biosensors: Throwing Color Lights on Molecular and Cellular Processes. Current Protein & Peptide Science, 9(4), 338–369.