Part:BBa_K4156101
pLldR
An Operon unit consisting of a collection of LldR-regulated promoters, and is capable of responding to lactate concentration.
Usage and Biology
Llprd operon is composed of the lldR regulatory protein ( BBa_K1847001 ), the terminator B0012, and the LldPRD promoter（ BBa_M36021 ）. At the LldPRD promoter, there are two operator sites O1 and O2 , which are able to bind to the lldR protein and block subsequent gene transcription. The presence of lactic acid molecules can unblock this blocking phenomenon. Thus, the Llprd operon can specifically respond to lactate and activate downstream genes. In our experiments, we used Llprd operon to adapt to the high lactate characteristics of the tumor cell microenvironment. Enabling our therapeutic strains to rapidly target to tumor cells and achieve targeted therapy.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 688
- 1000COMPATIBLE WITH RFC[1000]
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