Tag

Part:BBa_K4165020

Designed by: Esraa Elmligy   Group: iGEM22_CU_Egypt   (2022-09-30)
Revision as of 19:39, 9 October 2022 by Esraa Elmligy (Talk | contribs)


6x Histidine tag

This part encodes 6x Histidine tag used for purification and characterization of proteins

Usage and Biology

His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe (1). Parts of our systems are fused with his tag for purification and pull-down assay.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.


[edit]
Categories
Parameters
None