Part:BBa_K4165011

UBE2N

Ubiquitin-conjugating E2 ligase has a role in the ubiquitination cascade for protein degradation.

Usage and Biology

encodes a member of the E2 ubiquitin-conjugating enzyme family and is involved in the ubiquitin-proteasome pathway, this ligase will be used in the project in the Trim21 system as a type of ligase essential for the ubiquitin cascade

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 221
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Refrences

1- Komander D. (2009). The emerging complexity of protein ubiquitination. Biochemical Society transactions, 37(Pt 5), 937–953. https://doi.org/10.1042/BST0370937

2- David, Y., Ziv, T., Admon, A., & Navon, A. (2010). The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines. The Journal of biological chemistry, 285(12), 8595–8604. https://doi.org/10.1074/jbc.M109.089003

3- Li, J., Xu, C., Zhang, J., Jin, C., Shi, X., Zhang, C., ... & Xu, L. (2021). Identification of miRNA-target gene pairs in the parietal and frontal lobes of the brain in patients with Alzheimer’s Disease using bioinformatic analyses. Neurochemical Research, 46(4), 964-979.